Recombinant Human SFRS Protein Kinase 1, GST-His

• Cat.No.: SRPK1-713H
• Product Overview: Recombinant Human SRPK1, Amino acids M1-S655, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 104,220 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-655 a.a.
• Description: SRPK1 is a Ser/Thr protein kinase that has sequence and function similar to SRPK2. In human cancer cells, it was observed that inactivation of SRPK1 induces cellular resistance to anticancer drugs such as cisplatin and bleomycin. SRPK1 phosphorylates serine/arginine-rich (SR) proteins, such as splicing factors ASF/SF2, SC35, and SRp55, in their arginine/serine-rich (RS) domains in vitro.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: SRPK1 was confirmed as human SRPK1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.250 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 91 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: SRPK1 SFRS protein kinase 1 [ Homo sapiens ]
• Synonyms: SRPK1; SFRS protein kinase 1; SFRSK1; SR protein kinase 1; EC 2.7.11.1; SR-protein-specific kinase 1; Serine/arginine-rich protein-specific kinase 1; Serine/threonine-protein kinase SRPK1
• Gene ID: 6732
• mRNA Refseq: NM_003137
• Protein Refseq: NP_003128
• MIM: 601939
• UniProt ID: Q96SB4
• Chromosome Location: 6p21.3-p21.2
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity