Recombinant Human SRPK1 293 Cell Lysate

Description :	Antigen standard for SFRS protein kinase 1 (SRPK1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	SRPK1 SRSF protein kinase 1 [ Homo sapiens ]
Official Symbol	SRPK1
Synonyms	SRPK1; SRSF protein kinase 1; SFRS protein kinase 1; serine/arginine rich splicing factor kinase 1; SFRSK1; SR protein kinase 1; SR-protein-specific kinase 1; serine/threonine-protein kinase SRPK1; serine/arginine-rich splicing factor kinase 1;
Gene ID	6732
mRNA Refseq	NM_003137
Protein Refseq	NP_003128
MIM	601939
UniProt ID	Q96SB4
Chromosome Location	6p21.31
Pathway	Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; mRNA processing, organism-specific biosystem;