Recombinant Human SFN protein, GST-tagged

• Cat.No.: SFN-3857H
• Product Overview: Recombinant Human SFN protein(NP_006133.1)(Met1-Ser248), fused with GST tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: Met1-Ser248
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10mM GSH, 25% glycerol, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SFN/GST chimera consists of 480 amino acids and has a predicted molecular mass of 50.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SFN stratifin [ Homo sapiens ]
• Official Symbol: SFN
• Synonyms: SFN; stratifin; 14-3-3 protein sigma; 14 3 3 sigma; YWHAS; 14-3-3 sigma; epithelial cell marker protein 1
• Gene ID: 2810
• mRNA Refseq: NM_006142
• Protein Refseq: NP_006133
• MIM: 601290
• UniProt ID: P31947