Recombinant Human SF3B1 protein, His-tagged

• Cat.No.: SF3B1-1708H
• Product Overview: Recombinant Human SF3B1 aa. (Leu1038~Leu1304) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu1038~Leu1304
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >93%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 7.2

Gene Information

• Gene Name: SF3B1 splicing factor 3b subunit 1 [ Homo sapiens (human) ]
• Official Symbol: SF3B1
• Synonyms: SF3B1; splicing factor 3b subunit 1; MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155; splicing factor 3B subunit 1; pre-mRNA processing 10; pre-mRNA splicing factor SF3b, 155 kDa subunit; spliceosome-associated protein 155; splicing factor 3b, subunit 1, 155kDa
• Gene ID: 23451
• mRNA Refseq: NM_012433.3
• Protein Refseq: NP_036565.2
• UniProt ID: O75533