Recombinant Human SF3A1 protein, His-tagged

• Cat.No.: SF3A1-3708H
• Product Overview: Recombinant Human SF3A1 protein(443-793 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 443-793 aa
• AA Sequence: DRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: SF3A1 splicing factor 3a, subunit 1, 120kDa [ Homo sapiens ]
• Official Symbol: SF3A1
• Synonyms: SF3A1; splicing factor 3a, subunit 1, 120kDa; splicing factor 3a, subunit 1, 120kD; splicing factor 3A subunit 1; Prp21; PRPF21; SAP114; SF3a120; SAP 114; pre-mRNA processing 21; splicing factor 3 subunit 1; spliceosome associated protein 114; spliceosome-associated protein 114; pre-mRNA splicing factor SF3a (120 kDa subunit); PRP21; SF3A120
• Gene ID: 10291
• mRNA Refseq: NM_001005409
• Protein Refseq: NP_001005409
• MIM: 605595
• UniProt ID: Q15459