Recombinant Human SF3A1 293 Cell Lysate

• Cat.No.: SF3A1-1920HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SF3A1 splicing factor 3a, subunit 1, 120kDa [ Homo sapiens ]
• Official Symbol: SF3A1
• Synonyms: SF3A1; splicing factor 3a, subunit 1, 120kDa; splicing factor 3a, subunit 1, 120kD; splicing factor 3A subunit 1; Prp21; PRPF21; SAP114; SF3a120; SAP 114; pre-mRNA processing 21; splicing factor 3 subunit 1; spliceosome associated protein 114; spliceosome-associated protein 114; pre-mRNA splicing factor SF3a (120 kDa subunit); PRP21; SF3A120
• Gene ID: 10291
• mRNA Refseq: NM_001005409
• Protein Refseq: NP_001005409
• MIM: 605595
• UniProt ID: Q15459
• Chromosome Location: 22q12.2
• Pathway: Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U2-snRNP, organism-specific biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem
• Function: RNA binding; protein binding