Recombinant Human SETMAR, GST-tagged

• Cat.No.: SETMAR-6966H
• Product Overview: Recombinant full-length human SETMAR was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• ProteinLength: Full length
• Description: SETMAR or SET domain and mariner transposase fusion gene is a nonhomologous end-joining repair protein that regulates genomic integration of exogenous DNA by opening chromatin and facilitating joining of DNA ends. SETMAR has histone methyltransferase activity and methylates Lys-4 and Lys-36 of histone H3. SETMAR also has DNA nicking activity and may play a role in DNA repair. Human Pso4 forms a stable complex with SETMAR that regulates Metnase function in DNA repair. SETMAR has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5-terminal inverted repeats (TIRs) of the Hsmar1 element.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~66-69 kDa
• Purity: >85%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2 μg/μl

Gene Information

• Gene Name: SETMAR SET domain and mariner transposase fusion gene [ Homo sapiens ]
• Official Symbol: SETMAR
• Synonyms: SETMAR; SET domain and mariner transposase fusion gene; histone-lysine N-methyltransferase SETMAR; metnase; hsMar1; SET domain and mariner transposase fusion gene-containing protein; METNASE
• Gene ID: 6419
• mRNA Refseq: NM_001243723
• Protein Refseq: NP_001230652
• MIM: 609834
• UniProt ID: Q53H47
• Chromosome Location: 3p26.2
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: endonuclease activity; histone-lysine N-methyltransferase activity; hydrolase activity; metal ion binding; methyltransferase activity; protein binding; protein homodimerization activity; structure-specific DNA binding; structure-specific DNA binding; transferase activity; transposase activity; zinc ion binding