Recombinant Human SET Domain Containing (lysine methyltransferase) 7, GST-tagged

• Cat.No.: SETD7-1630H
• Product Overview: Recombinant full-length human SETD7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: SETD7 is a lysine methyltransferase which can methylates lysine-4 in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300. SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability.
• Applications: Western Blot
• Molecular Weight: 74 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25 % glycerol.
• Purity: > 95 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Lysine degradation; p53 pathway

Gene Information

• Gene Name: SETD7 SET domain containing (lysine methyltransferase) 7 [ Homo sapiens ]
• Official Symbol: SETD7
• Synonyms: SETD7; SET domain containing (lysine methyltransferase) 7; KMT7; SET7; SET9; SET7/9; FLJ21193; KIAA1717; histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; OTTHUMP00000164543; OTTHUMP00000220049; lysine N-methyltransferase 7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; EC 2.1.1.43; Histone-lysine N-methyltransferase SETD7; Histone H3-K4 methyltransferase SETD7; Lysine N-methyltransferase 7; SET domain-containing protein 7
• Gene ID: 80854
• mRNA Refseq: NM_030648
• Protein Refseq: NP_085151
• MIM: 606594
• UniProt ID: Q8WTS6
• Chromosome Location: 4q31.1
• Function: histone-lysine N-methyltransferase activity; methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity; transferase activity