Recombinant Human Serum/Glucocorticoid Regulated Kinase Family, Member 3, GST-tagged

• Cat.No.: SGK3-966H
• Product Overview: A DNA sequence encoding the human SGK3 ( Q96BR1-1 ) ( Met 1 - Leu 496 ) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Human
• Tag: GST
• Protein Length: 1-496 a.a.
• Description: SGK3 is a cytoplasmic vesicle protein which belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. SGK3 contains one AGC-kinase C-terminal domain, one protein kinase domain and one PX (phox homology) domain. SGK3 is needed to be phosphorylated for its full activation.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Met
• Molecular Mass: The recombinant human SGK3/GST chimera consists of 733 amino acids and has a calculated molecular mass of 85 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Pathways: Insulin Signaling

Gene Information

• Gene Name: SGK3 serum/glucocorticoid regulated kinase family, member 3 [ Homo sapiens ]
• Official Symbol: SGK3
• Synonyms: SGK3; serum/glucocorticoid regulated kinase family, member 3; CISK; SGK2; SGKL; DKFZp781N0293; serine/threonine-protein kinase Sgk3; OTTHUMP00000226962; OTTHUMP00000226963; OTTHUMP00000226965; OTTHUMP00000226966; OTTHUMP00000226969; cytokine-independent survival kinase; EC 2.7.11.1; serum/glucocorticoid regulated kinase-like; Serine/threonine-protein kinase Sgk3; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like
• Gene ID: 23678
• mRNA Refseq: NM_001033578
• Protein Refseq: NP_001028750
• MIM: 607591
• UniProt ID: Q96BR1
• Chromosome Location: 8q12