Recombinant Human Serum/Glucocorticoid Regulated Kinase 2

• Cat.No.: SGK2-659H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C, and its catalytic domain is most similar to protein kinase B (PKB). SGK2, like the other two isoforms SGK1 and SGK3, is stimulated by insulin and insulin-like growth factor-1 (IGF-1), and has been shown to enhance Na(+)/K(+)-ATPase activity in a variety of cells.
• Application: Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
• Biochem/physiol Actions: SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na+/K+-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na+ channel ENaC activity.
• Physical Form: Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.
• Assay: ≥75% (SDS-PAGE).
• Form: aqueous glycerol solution.
• Mol Wt: protein apparent mol wt ~66 kDa.
• Storage Temp: −70°C.

Gene Information

• Gene Name: SGK2 serum/glucocorticoid regulated kinase 2 [ Homo sapiens ]
• Synonyms: SGK2; serum/glucocorticoid regulated kinase 2; H-SGK2; dJ138B7.2; OTTHUMP00000031705; EC 2.7.11.1; OTTHUMP00000031702; OTTHUMP00000031703; Serum/glucocorticoid-regulated kinase 2; Serine/threonine-protein kinase Sgk2
• Gene ID: 10110
• mRNA Refseq: NM_016276
• Protein Refseq: NP_057360
• MIM: 607589
• UniProt ID: Q9HBY8
• Chromosome Location: 20q13.2
• Function: ATP binding; nucleotide binding; potassium channel regulator activity; protein serine/threonine kinase activity; sodium channel regulator activity; transferase activity