Recombinant Human Serine Hydroxymethyltransferase 1, His-tagged

• Cat.No.: SHMT1-1542H
• Product Overview: Recombinant human SHMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Molecular Weight: 55.2 kDa (503aa)
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTMPVNGAHK DADLWSSHDK MLAQPLKDSD VEVYNIIKKE SNRQRVGLEL IASENFASRA VLEALGSCLN NKYSEGYPGQ RYYGGTEFID ELETLCQKRA LQAYKLDPQC WGVNVQPYSG SPANFAVYTA LVEPHGRIMG LDLPDGGHLT HGFMTDKKKI SATSIFFESM PYKVNPDTGY INYDQLEENA RLFHPKLIIA GTSCYSRNLE YARLRKIADE NGAYLMADMA HISGLVAAGV VPSPFEHCHV VTTTTHKTLR GCRAGMIFYR KGVKSVDPKT GKEILYNLES LINSAVFPGL QGGPHNHAIA GVAVALKQAM TLEFKVYQHQ VVANCRALSE ALTELGYKIV TGGSDNHLIL VDLRSKGTDG GRAEKVLEAC SIACNKNTCP GDRSALRPSG LRLGTPALTS RGLLEKDFQK VAHFIHRGIE LTLQIQSDTG VRATLKEFKE RLAGDKYQAA VQALREEVES FASFFPLPGL PDF
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Carnitine synthesis; Cyanoamino acid metabolism; Glycine, serine and threonine metabolism; Metabolic pathways; Metabolism of amino acids and derivatives; Metabolism of folate and pterines; Metabolism of vitamins and cofactors; Metabolism of water-soluble vitamins and cofactors; One Carbon Metabolism; One carbon pool by folate; Validated targets of C-MYC transcriptional activation

Gene Information

• Gene Name: SHMT1 serine hydroxymethyltransferase 1 (soluble) [ Homo sapiens ]
• Official Symbol: SHMT1
• Synonyms: SHMT1; SHMT; CSHMT; MGC15229; MGC24556; serine hydroxymethyltransferase 1 (soluble); serine hydroxymethyltransferase, cytosolic; 14 kDa protein; serine methylase; OTTHUMP00000065139; OTTHUMP00000065140; OTTHUMP0000006514;1 glycine hydroxymethyltransferase; cytoplasmic serine hydroxymethyltransferase
• Gene ID: 6470
• mRNA Refseq: NM_004169
• Protein Refseq: NP_004160
• MIM: 182144
• UniProt ID: P34896
• Chromosome Location: 17p11.2
• Function: L-allo-threonine aldolase activity; amino acid binding; glycine hydroxymethyltransferase activity; protein homodimerization activity; pyridoxal phosphate binding; transferase activity