Recombinant Human Sequestosome 1, His-tagged

• Cat.No.: SQSTM1-2358H
• Product Overview: Recombinant human SQSTM1 protein, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 39.7 kDa (364aa).

Specification

• Cat. No.: SQSTM1-2358H
• Description: SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of the sequestosome 1 protein commonly cause Paget's disease since the UBA is necessary for aggregate sequestration and cell survival.
• Source: E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MAMSYVKDDI FRIYIKEKKE CRRDHRPPCA QEAPRNMVHP NVICDGCNGP VVGTRYKCSV CPDYDLCSVC EGKGLHRGHT KLAFPSPFGH LSEGFSHSRW LRKVKHGHFG WPGWEMGPPG NWSPRPPRAG EARPGPTAES ASGPSEDPSV NFLKNVGESV AAALSPLGIE VDIDVEHGGK RSRLTPVSPE SSSTEEKSSS QPSSCCSDPS KPGGNVEGAT QSLAEQMRKI ALESEGRPEE QMESDNCSGG DDDWTHLSSK EVDPSTGELQ SLQMPESEGP SSLDPSQEGP TGLKEAALYP HLPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KHPPPLLEHH HHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SQSTM1 sequestosome 1 [ Homo sapiens ]
• Synonyms: sequestosome 1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; SQSTM1; sequestosome-1; EBIAP; EBI3-associated protein p60; oxidative stress induced like; ubiquitin-binding protein p62; EBI3-associated protein of 60 kDa; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;Ubiquitin-binding protein p62; Paget disease of bone 3; ORCA; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; P62
• Gene ID: 8878
• mRNA Refseq: NM_001142298
• Protein Refseq: NP_001135770
• MIM: 601530
• UniProt ID: Q13501
• Chromosome Location: 5q35
• Pathway: Signalling by NGF
• Function: receptor tyrosine kinase binding; SH2 domain binding; metal ion binding; protein binding; protein kinase C binding; protein kinase binding; ubiquitin binding; zinc ion binding