Recombinant Human SEMA3A Protein, His-tagged

• Cat.No.: SEMA3A-1901H
• Product Overview: Recombinant Human SEMA3A Protein (Arg31~Cys141) is produced by E. coli expression system. This protein is fused with a His tag at the N-terminal.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300
• Molecular Mass: Predicted Molecular Mass: 16.6 kDa; Accurate Molecular Mass: 16 kDa(Analysis of differences refer to the manual)
• Protein length: Arg31~Cys141
• AA Sequence: RLKLSYKEMLESNNVITFNCLANSS SYHTFLLDEERSRLYVGAKDHIFSF DLVNIKDFQKIVWPVSYTRRDECKW AGKDILKECANFIKVLKAYNQTHLY ACGTGAFHPIC
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SEMA3A sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Homo sapiens ]
• Official Symbol: SEMA3A
• Synonyms: SEMA3A; SEMAD; semaphorin-3A; coll 1; Hsema I; sema III; SEMA1; SemD; collapsin 1; semaphorin D; semaphorin L; semaphorin III; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243
• Gene ID: 10371
• mRNA Refseq: NM_006080
• Protein Refseq: NP_006071
• MIM: 603961
• UniProt ID: Q14563