Recombinant Human Selectin L, Fc Chimera

• Cat.No.: SELL-451H
• Product Overview: Recombinant Human selectin L encoding the signal peptide, pro-peptide and extracellular domain of human L-Selectin (aa 1-343) was expressed in modifiedhuman 293 cells. It was fused to the Fc region of human IgG1 (aa 93-330).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-343 a.a.
• Description: L-Selectin (CD62L) is a type I membrane protein expressed on the surface of T cells, B cells, monocytes, neutrophils, macrophages, eosinophils and NK cells. L-Selectin mediates entry of lymphocytes into secondary lymphoid tissue by adherence to endothelial cells of high endothelial venules (HEV). L-Selectin has been considered as a suitable target for treatment or prevention of inflammatory diseases such as asthma, rheumatoid arthritis, inflammatory bowel disease, allergies and atherosclerosis.
• Amino Acid Sequence: WTYHYSEKPMNWQRA RRFCRDNYTDLVA IQNKAEIEYLEKTL PFSRSYYWIGIRKI GGIWTWVGTNKS LTEEAENWGDGE PNNKKNKEDCVE IYIKRNKDAGKWN DDA CHK LKAALCYTASCQPWS CSGHGECVEIIN NYTCNCDVGY YGPQCQFVIQ CEP LEA PEL G T MDCTHPLGNF SFS QCAF SCSEGTNL TGIEETTC GPFGNWS SPE PTCQV IQC E P LSA PDLGIMNC SH PLASF SFTSACT FICSEGTEL IGKKKTICES SG IW SNPSPI CQ KLDKSFS MIKEGDW PKVDKKV EPKSCDK THTCPPC PAP ELLG GPSVFLF PPKPKDT LMISRTPE VTCVVVD VSHEDPE VKFNWYV DGV EVH N AKTKPREE QYNSTYR VVS VLTVL HQDWLNG KEYKCRV SNKALPA PIEKTISKA KGQPREP QVYTLPP SRDELTK NQVSLTCLV KGFYPSDIA VEWESNG QPENNYKT TPPVLDSD GSFFLYSKL TVDKS RWQ QGNV FSCSV MHEAL HNHYT QKSLS LS PG K.
• Molecular Mass: L-Selectin-Fc Chimera migrates as a broad band between 75 and 105 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified L-Selectin-Fc Chimera that has a predicted molecular mass of 59.9kDa.
• PI: L-Selectin-Fc Chimera separates into a number of isoforms with a pI between 4.6 and 6.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified L-Selectin-Fc Chimera that has a predicted pI of 5.92.
• % Carbohydrate: Purified L-Selectin-Fc Chimera consists of 20-45% carbohydrate by weight.
• Glycosylation: L-Selectin-Fc Chimera has N-linked and possibly O-Linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: SELL selectin L [ Homo sapiens ]
• Synonyms: SELL; selectin L; CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; TQ1; CD62 antigen-like family member L; L-selectin; gp90-MEL; leukocyte adhesion molecule-1; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; CD62L antigen; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor
• Gene ID: 6402
• mRNA Refseq: NM_000655
• Protein Refseq: NP_000646
• MIM: 153240
• UniProt ID: P14151
• Chromosome Location: 1q23-q25
• Pathway: Cell adhesion molecules (CAMs); Hemostasis; Signaling in Immune system
• Function: glycosphingolipid binding; heparin binding; protein binding; sugar binding