Recombinant human SEC61B, His-tagged

• Cat.No.: SEC61B-2454H
• Product Overview: Recombinant human SEC61B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Molecular Mass: 9.4kDa (93aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPGPTPS GTNVGSSGRS PSKAVAARAA GSTVRQRKNA SCGTRSAGRT TSAGTGGMWR FYTEDSPGLKVGP
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SEC61B Sec61 beta subunit [ Homo sapiens ]
• Official Symbol: SEC61B
• Synonyms: SEC61B; Sec61 beta subunit; protein transport protein Sec61 subunit beta; Sec61 complex, beta subunit; protein translocation complex beta; protein transport protein SEC61 beta subunit
• Gene ID: 10952
• mRNA Refseq: NM_006808
• Protein Refseq: NP_006799
• MIM: 609214
• UniProt ID: P60468
• Chromosome Location: 9q22.32-q31.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; ER-Phagosome pathway, organism-specific biosystem; Gene Expression, organism-specific biosystem; Immune System, organism-specific biosystem
• Function: epidermal growth factor binding; protein binding