Recombinant Human SCNN1B 293 Cell Lysate

• Cat.No.: SCNN1B-2028HCL

Specification

• Description: Antigen standard for sodium channel, nonvoltage-gated 1, beta (SCNN1B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SCNN1B sodium channel, non-voltage-gated 1, beta subunit [ Homo sapiens ]
• Official Symbol: SCNN1B
• Synonyms: SCNN1B; sodium channel, non-voltage-gated 1, beta subunit; sodium channel, non voltage gated 1, beta , sodium channel, nonvoltage gated 1, beta; amiloride-sensitive sodium channel subunit beta; ENaCbeta; Liddle syndrome; beta-ENaC; beta-NaCH; epithelial Na(+) channel subunit beta; epithelial sodium channel beta-2 subunit; epithelial sodium channel beta-3 subunit; sodium channel, nonvoltage-gated 1, beta; nasal epithelial sodium channel beta subunit; nonvoltage-gated sodium channel 1 subunit beta; amiloride-sensitive sodium channel subunit beta 1; BESC1; ENaCb; SCNEB
• Gene ID: 6338
• mRNA Refseq: NM_000336
• Protein Refseq: NP_000327
• MIM: 600760
• UniProt ID: P51168
• Chromosome Location: 16p12.2-p12.1
• Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Taste transduction, organism-specific biosystem; Taste transduction, conserved biosystem
• Function: WW domain binding; ion channel activity; ligand-gated sodium channel activity; protein binding