Recombinant Human SCGN, His-tagged

• Cat.No.: SCGN-3740H
• Product Overview: Total 286 AA. MW: 33.3 kDa (calculated). N-terminal His-tag, 10exrea AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry O76038.

Specification

• Cat. No.: SCGN-3740H
• Description: Secretagogin is a protein that in humans is encoded by the SCGN gene. The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation.
• Source: E. coli
• Species: Human
• Form: Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 20mM NaCl, pH 7.5.
• Research topic: Diabetology ?C Autoimmunity; Neural tissue damage markers; Oncology; Pancreatic regulatory molecules
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS MDSSREPTLG RLDAAGFWQV WRRFDADEKG YIEEKELDAF FLHMLMKLGT DDTVMKANLH KVKQQFMTTQ DASKDGRIRM KELAGMFLSE DENFLLLFRR ENPLDSSVEF MQIWRKYDAD SSGFISAAEL RNFLRDLFLH HKKAISEAKL EEYTGTMMKI FDRNKDGRLD LNDLARILAL QENFLLQFKM DACSTEERKR DFEKIFAYYD VSKTGALEGP EVDGFVKDMM ELVQPSISGV DLDKFREILL RHCDVNKDGK IQKSELALCL GLKINP
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: SCGN secretagogin, EF-hand calcium binding protein [ Homo sapiens ]
• Official Symbol: SCGN
• Synonyms: SCGN; secretagogin, EF-hand calcium binding protein; SEGN; CALBL; SECRET; setagin; DJ501N12.8; secretagogin; OTTHUMP00000016124
• Gene ID: 10590
• mRNA Refseq: NM_006998
• Protein Refseq: NP_008929
• MIM: 609202
• UniProt ID: O76038
• Chromosome Location: 6p22.3-p22.1
• Function: calcium ion binding