Recombinant Human S1PR4 293 Cell Lysate

• Cat.No.: S1PR4-2083HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for sphingosine-1-phosphate receptor 4 (S1PR4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: S1PR4 sphingosine-1-phosphate receptor 4 [ Homo sapiens ]
• Official Symbol: S1PR4
• Synonyms: S1PR4; sphingosine-1-phosphate receptor 4; EDG6, endothelial differentiation, G protein coupled receptor 6 , endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 6; sphingosine 1-phosphate receptor 4; S1P receptor 4; S1P receptor Edg-6; sphingosine 1-phosphate receptor Edg-6; endothelial differentiation G-protein coupled receptor 6; endothelial differentiation, G protein coupled receptor 6; endothelial differentiation, G-protein-coupled receptor 6; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 6; EDG6; LPC1; S1P4; SLP4
• Gene ID: 8698
• mRNA Refseq: NM_003775
• Protein Refseq: NP_003766
• MIM: 603751
• UniProt ID: O95977
• Chromosome Location: 19p13.3
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Lysosphingolipid and LPA receptors, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
• Function: G-protein coupled receptor activity; lipid binding; receptor activity; signal transducer activity; sphingosine-1-phosphate receptor activity