Recombinant Human S100A6 protein, His-tagged

Source :	E. coli
Species :	Human
Tag :	His
Protein length :	Met1~Gly90
Form :	20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
Bio-activity :	S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE(Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure, and this effect was in a dose dependent manner.
Molecular Mass :	16kDa
Purity :	>92%
Storage :	Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution :	Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Name	S100A6 S100 calcium binding protein A6 [ Homo sapiens ]
Official Symbol	S100A6
Synonyms	S100A6; S100 calcium binding protein A6; CACY, S100 calcium binding protein A6 (calcyclin) , S100 calcium binding protein A6 (calcyclin); protein S100-A6; 2A9; CABP; PRA; MLN 4; calcyclin; growth factor-inducible protein 2A9; prolactin receptor-associated protein; S100 calcium-binding protein A6 (calcyclin); 5B10; CACY
Gene ID	6277
mRNA Refseq	NM_014624
Protein Refseq	NP_055439
MIM	114110
UniProt ID	P06703