Recombinant Human S100A12, His-tagged

• Cat.No.: S100A12-30229TH
• Product Overview: Recombinant full length Human S100A12 with an N terminal His tag; 101 amino acids with tag, predicted MWt: 11.6 kDa.

Specification

• Description: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
• Protein length: 91 amino acids
• Conjugation: HIS
• Molecular Weight: 11.600kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Monocytes and lymphocytes.
• Form: Lyophilised:Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter the product by an appropriate sterile filter before using it in the cell culture.
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50mM Sodium chloride, 20mM Tris, pH 7.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MKHHHHHHAS TKLEEHLEGI VNIFHQYSVR KGHFDTLSKG ELKQLLTKEL ANTIKNIKDK AVIDEIFQGL DANQDEQVDFQEFISLVAIA LKAAHYHTHK E
• Sequence Similarities: Belongs to the S-101 family.Contains 2 EF-hand domains.

Gene Information

• Gene Name: S100A12 S100 calcium binding protein A12 [ Homo sapiens ]
• Official Symbol: S100A12
• Synonyms: S100A12; S100 calcium binding protein A12; S100 calcium binding protein A12 (calgranulin C) , S100 calcium binding protein A12 (calgranulin C); protein S100-A12; CAAF1; CAGC; CGRP; ENRAGE; MRP6; p6
• Gene ID: 6283
• mRNA Refseq: NM_005621
• Protein Refseq: NP_005612
• MIM: 603112
• Uniprot ID: P80511
• Chromosome Location: 1q21
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem
• Function: RAGE receptor binding; RAGE receptor binding; calcium ion binding; protein binding; zinc ion binding