Recombinant Human RSPO1 protein, For Organoid Culture

• Cat.No.: RSPO1-2943H
• Product Overview: Recombinant Human RSPO1 protein(Q2MKA7)(Ser21-Ala263) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: Ser21-Ala263
• Tag: Non
• Form: Lyophilized from sterile PBS, pH 7.4, 3% trehalose.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293 reporter cells. The ED50 for this effect is 0.5-5.0ng/mL in the presence of 20 ng/mL Recombinant Wnt Surrogate Fc Chimera Protein.
• Molecular Mass: 25.6 kDa (monomer)
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions.
• Storage: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below: To the date of expiration, -20°C to -80°C as supplied. 3 months, -20°C to -80°C under sterile conditions after reconstitution. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7