Recombinant Human RSPO1 Protein

• Cat.No.: RSPO1-34H
• Product Overview: Recombinant human R-spondin 1 protein without tag was expressed in E. coli.
  Animal-free (AOF) and carrier protein-free.
  Mass spectrometry: single species with expected mass.
  Analytical reversed-phase: single sharp peak.
  Recovery from stock vial: >95%

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
• Molecular Mass: 13 kDa
• Endotoxin: <0.005 EU/μg protein (below level of detection)
• Applications: Neuronal Differentiation
• Storage: Resuspend in 10mM HCl at >100 μg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade
• Storage Buffer: Lyophilized from acetonitrile, TFA

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens (human) ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7