Active Recombinant Human RSPO1 Protein, Pre-aliquoted

Specification
Gene Information
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Description :	This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
Source :	CHO
Species :	Human
Bio-activity :	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a.
Molecular Mass :	Predicted Molecular Mass:25.6 kDa SDS-PAGE: 39 kDa, reducing conditions
Protein length :	Arg31-Ala263
N-terminal Sequence Analysis :	Ser21 & Arg31
Endotoxin :	< 0.10 EU/μg of the protein by the LAL method.
Purity :	> 90% by SDS-PAGE under reducing conditions and visualized by silver stain.
Stability :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 centigrade as supplied. • 1 month at 2-8 centigrade under sterile conditions after reconstitution. • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
Reconstitution :	For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll Pre-aliquoted directly into cell culture medium.
Shipping :	The product is shipped at ambient temperature.

Gene Name	RSPO1 R-spondin 1 [ Homo sapiens (human) ]
Official Symbol	RSPO1
Synonyms	RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
Gene ID	284654
mRNA Refseq	NM_001038633
Protein Refseq	NP_001033722
MIM	609595
UniProt ID	Q2MKA7