Active Recombinant Human RSPO1 Protein, Pre-aliquoted

• Cat.No.: RSPO1-28H
• Product Overview: Recombinant Human RSPO1 Protein, Arg31 - Ala263, was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Protein Length: Arg31-Ala263
• Description: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a.
• Molecular Mass: Predicted Molecular Mass:25.6 kDa
  SDS-PAGE: 39 kDa, reducing conditions
• N-terminal Sequence Analysis: Ser21 & Arg31
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll Pre-aliquoted directly into cell culture medium.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens (human) ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7