Recombinant Human RRAGB Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RRAGB-5293H
• Product Overview: RRAGB MS Standard C13 and N15-labeled recombinant protein (NP_006055) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified.
• Molecular Mass: 40.2 kDa
• AA Sequence: MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RRAGB Ras-related GTP binding B [ Homo sapiens (human) ]
• Official Symbol: RRAGB
• Synonyms: RRAGB; Ras-related GTP binding B; ras-related GTP-binding protein B; rag B; GTP-binding protein ragB; RAGB; bA465E19.1
• Gene ID: 10325
• mRNA Refseq: NM_006064
• Protein Refseq: NP_006055
• MIM: 300725
• UniProt ID: Q5VZM2