Recombinant Human RPTOR Protein, His-tagged

• Cat.No.: RPTOR-2666H
• Product Overview: Recombinant Human RPTOR Protein (Asn1020-Arg1335) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.1 kDa
  Accurate Molecular Mass: 37 kDa
• Protein length: Asn1020-Arg1335
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: RPTOR regulatory associated protein of MTOR, complex 1 [ Homo sapiens (human) ]
• Official Symbol: RPTOR
• Synonyms: RPTOR; regulatory associated protein of MTOR, complex 1; regulatory-associated protein of mTOR; KIAA1303; KOG1; Mip1; raptor; regulatory associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
• Gene ID: 57521
• mRNA Refseq: NM_001163034
• Protein Refseq: NP_001156506
• MIM: 607130
• UniProt ID: Q8N122