Recombinant Human RPIA, His-tagged

• Cat.No.: RPIA-31148TH
• Product Overview: Recombinant full length Human RPIA with N terminal His tag; 331 amino acids with tag, Predicted MWt 35.4 kDa.

Specification

• Description: The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18.
• Protein length: 311 amino acids
• Conjugation: HIS
• Molecular Weight: 35.400kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.03% DTT, 40% Glycerol, 1.17% Sodium chloride, 0.06% EDTA, 0.004% PMSF
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMQRPGPFSTLYGRVLAPLPG RAGGAASGGGGNSWDLPGSHVRLPGRAQSGTRGGAGNTST SCGDSNSICPAPSTMSKAEEAKKLAGRAAVENHVRNNQVL GIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLIL QYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLT QEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPM AYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDW KFDRVHKWSEVNTAIKMIPGVVDTGLFINMAERVYFGMQD GSVNMREKPFC
• Sequence Similarities: Belongs to the ribose 5-phosphate isomerase family.

Gene Information

• Gene Name: RPIA ribose 5-phosphate isomerase A [ Homo sapiens ]
• Official Symbol: RPIA
• Synonyms: RPIA; ribose 5-phosphate isomerase A; ribose-5-phosphate isomerase; ribose 5 phosphate epimerase
• Gene ID: 22934
• mRNA Refseq: NM_144563
• Protein Refseq: NP_653164
• MIM: 180430
• Uniprot ID: P49247
• Chromosome Location: 2p11.2
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem; Pentose Phosphate Pathway, organism-specific biosystem; Pentose phosphate pathway, organism-specific biosystem; Pentose phosphate pathway, conserved biosystem
• Function: isomerase activity; monosaccharide binding; ribose-5-phosphate isomerase activity