Recombinant Human RNMT, His-tagged

Cat. No. :	RNMT-3535H
Description :	RNMT is a widelyexpressed nuclear protein that belongs to the mRNA cap methyltransferasefamily. Cap-dependent mRNA translation requires the methylation of the mRNAguanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group fromAdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppNterminated RNA.
Application :	SDS-PAGE
Form :	Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl.
Molecular Weight :	57.0 kDa (496aa)
Purity :	> 90% by SDS-PAGE
Concentration :	0.5 mg/ml(determined by Bradford assay)
Sequences of aminoacids :	MGSSHHHHHHSSGLVPRGSHMANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVCRQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTGDGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEKVRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITADSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEASETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYEEKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVFAFEKQQ
Storage :	Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
OfficialSymbol :	RNMT

Gene Name	RNMT RNA (guanine-7- )methyltransferase [ Homo sapiens ]
Synonyms	RNMT; RNA (guanine-7- ) methyltransferase; mRNA cap guanine-N7methyltransferase; RG7MT1; MET; hCMT1c; KIAA0398;hMet; mRNA (guanine-N(7)-) methyltransferase; mRNA cap methyltransferase; EC2.1.1.56; DKFZp686H1252; OTTHUMP00000162509; mRNA (guanine-7-)methyltransferase
Gene ID	8731
mRNA Refseq	NM_003799
Protein Refseq	NP_003790
MIM	603514
UniProt ID	O43148
Chromosome Location	18p11.21
Pathway	Gene Expression; HIV Infection; HIV LifeCycle; Late Phase of HIV Life Cycle; Disease; RNA Pol II CTD phosphorylationand interaction with CE; RNA Polymerase II Transcription; Transcription ofthe HIV genome; mRNA Capping; mRNA processing; mRNA surveillance pathway
Function	RNA binding; mRNA(guanine-N7-)-methyltransferase activity; methyltransferase activity;transferase activity; protein binding

COA

Cat.No. :	RNMT-3535H
Product Overview :	Recombinant humanRNMT protein, fused to His-tag at N-terminus, was expressed in E. coliand purified by using conventional chromatography techniques.