Recombinant Human RNH1 protein, His & T7-tagged

• Cat.No.: RNH1-6967H
• Product Overview: Recombinant Human RNH1 aa. (Asp218~Glu444 (Accession # P13489)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.4kDa
• Protein length: Asp218~Glu444
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: RNH1 ribonuclease/angiogenin inhibitor 1 [ Homo sapiens (human) ]
• Official Symbol: RNH1
• Synonyms: RNH1; ribonuclease/angiogenin inhibitor 1; RAI; RNH; ribonuclease inhibitor; placental RNase inhibitor; placental ribonuclease inhibitor; testicular tissue protein Li 164
• Gene ID: 6050
• mRNA Refseq: NM_002939.3
• Protein Refseq: NP_002930.2
• UniProt ID: P13489