Recombinant Human RNASEH2A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RNASEH2A-4598H
• Product Overview: RNASEH2A MS Standard C13 and N15-labeled recombinant protein (NP_006388) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.
• Molecular Mass: 33.4 kDa
• AA Sequence: MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RNASEH2A ribonuclease H2 subunit A [ Homo sapiens (human) ]
• Official Symbol: RNASEH2A
• Synonyms: RNASEH2A; ribonuclease H2, subunit A; Aicardi Goutieres syndrome 4, ribonuclease H2, large subunit; ribonuclease H2 subunit A; AGS4; RNASEHI; RNHIA; RNHL; RNase H(35); RNase H2 subunit A; RNase HI large subunit; ribonuclease HI subunit A; ribonuclease HI large subunit; ribonuclease H2, large subunit; ribonuclease HI, large subunit; aicardi-Goutieres syndrome 4 protein; JUNB
• Gene ID: 10535
• mRNA Refseq: NM_006397
• Protein Refseq: NP_006388
• MIM: 606034
• UniProt ID: O75792