Recombinant Human RNASEH1 protein, MYC/DDK-tagged

• Cat.No.: RNASEH1-4321H
• Product Overview: Recombinant Human RNASEH1 fussed with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Myc&DDK
• Description: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes; two of them are on different locations of chromosome 17 and one of them is on chromosome 1q32.2.
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: RNASEH1 ribonuclease H1 [ Homo sapiens ]
• Official Symbol: RNASEH1
• Synonyms: RNASEH1; ribonuclease H1; RNase H1; ribonuclease H type II; RNH1; H1RNA
• Gene ID: 246243
• mRNA Refseq: NM_002936
• Protein Refseq: NP_002927
• MIM: 604123
• UniProt ID: O60930
• Chromosome Location: 2p25
• Pathway: DNA replication, organism-specific biosystem; DNA replication, conserved biosystem
• Function: RNA binding; endonuclease activity; hydrolase activity; magnesium ion binding; nucleic acid binding; protein homodimerization activity; ribonuclease H activity; ribonuclease activity