Recombinant Human ribonuclease, RNase A family, 7, His-tagged

• Cat.No.: RNASE7-548H
• Product Overview: RNASE7, 29-156aa, Human His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 29-156 a.a.
• Description: RNASE7 is one of the final RNase A superfamily ribonucleases. It was isolated from skin-derived stratum corneum. This protein exhibited potent ribonuclease activity and thus may contribute to the well known ribonuclease activity of human skin. It revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity against a vancomycin-resistant Enterococcus faecium. Recombinant human RNASE7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 1mM DTT
• Molecular Mass: 16.9 kDa(151aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF HLVPVHLDRV L
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RNASE7 ribonuclease, RNase A family, 7 [ Homo sapiens ]
• Official Symbol: RNASE7
• Synonyms: RNASE7; ribonuclease, RNase A family, 7; ribonuclease 7; SAP-2; RNase 7; skin-derived antimicrobial protein 2; MGC133220
• Gene ID: 84659
• mRNA Refseq: NM_032572
• Protein Refseq: NP_115961
• MIM: 612484
• UniProt ID: Q9H1E1
• Chromosome Location: 14q11.1
• Function: endonuclease activity; hydrolase activity; nucleic acid binding; pancreatic ribonuclease activity; ribonuclease activity