Recombinant Human RGS20 293 Cell Lysate

• Cat.No.: RGS20-2377HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for regulator of G-protein signaling 20 (RGS20), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RGS20 regulator of G-protein signaling 20 [ Homo sapiens ]
• Official Symbol: RGS20
• Synonyms: RGS20; regulator of G-protein signaling 20; regulator of G protein signalling 20; RGSZ1; ZGAP1; gz-GAP; g(z)GAP; regulator of G-protein signaling Z1; regulator of G-protein signalling 20; gz-selective GTPase-activating protein; regulator of G-protein signaling 20 variant 2; regulator of Gz-selective protein signaling 1
• Gene ID: 8601
• mRNA Refseq: NM_003702
• Protein Refseq: NP_003693
• MIM: 607193
• UniProt ID: O76081
• Chromosome Location: 8q11.23
• Pathway: Calcium Regulation in the Cardiac Cell, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G alpha (z) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem
• Function: GTPase activator activity