Recombinant Human RECQL 293 Cell Lysate

Description :	Antigen standard for RecQ protein-like (DNA helicase Q1-like) (RECQL), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	RECQL RecQ protein-like (DNA helicase Q1-like) [ Homo sapiens ]
Official Symbol	RECQL
Synonyms	RECQL; RecQ protein-like (DNA helicase Q1-like); ATP-dependent DNA helicase Q1; RecQ1; RecQL1; recQ protein-like 1; DNA-dependent ATPase Q1; DNA helicase, RecQ-like type 1; RECQL1;
Gene ID	5965
mRNA Refseq	NM_002907
Protein Refseq	NP_002898
MIM	600537
UniProt ID	P46063
Chromosome Location	12p12.1
Function	ATP binding; ATP-dependent 3-5 DNA helicase activity; ATP-dependent DNA helicase activity; DNA binding; DNA helicase activity; DNA strand annealing activity; hydrolase activity; nucleotide binding; protein binding;