Recombinant Human RD RNA Binding Protein, His-tagged

• Cat.No.: RDBP-3419H
• Product Overview: Recombinant human RDBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: RDBP-3419H
• Description: RDBP, also known as NELF-E, is a putative RNA binding protein. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions.
• Source: E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 45.4 kDa (400aa) confirmed by MALDI-TOF.
• Purity: > 85% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MLVIPPGLSE EEEALQKKFN KLKKKKKALL AL KK QSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TK NS GF KRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLY ES FV SS S DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPP RSR SRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRD RE RDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPF GN IIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: HIV Infection; Gene Expression; Transcription

Gene Information

• Gene Name: RDBP RD RNA binding protein [ Homo sapiens ]
• Synonyms: RD RNA binding protein; RD; RDP; D6S45; NELF-E; RDBP; negative elongation factor E; nuclear protein; OTTHUMP00000174687; OTTHUMP0-0000174689; OTTHUMP00000174710; RD RNA-binding protein; RNA-binding protein RD; major histocompatibility complex gene RD; negative elongation factor polypeptide E;NELFE
• Gene ID: 7936
• mRNA Refseq: NM_002904
• Protein Refseq: NP_002895
• MIM: 154040
• UniProt ID: P18615
• Chromosome Location: 6p21.3
• Function: RNA binding; nucleotide binding; protein binding