Recombinant Human RCC1 293 Cell Lysate

• Cat.No.: RCC1-2445HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for regulator of chromosome condensation 1 (RCC1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RCC1 regulator of chromosome condensation 1 [ Homo sapiens ]
• Official Symbol: RCC1
• Synonyms: RCC1; regulator of chromosome condensation 1; CHC1, chromosome condensation 1; regulator of chromosome condensation; cell cycle regulatory protein; SNHG3-RCC1 readthrough transcript; guanine nucleotide-releasing protein; CHC1; RCC1-I; SNHG3-RCC1
• Gene ID: 1104
• mRNA Refseq: NM_001048194
• Protein Refseq: NP_001041659
• MIM: 179710
• UniProt ID: P18754
• Chromosome Location: 1p35.3
• Pathway: Disease, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem; Host Interactions of HIV factors, organism-specific biosystem; Interactions of Rev with host cellular proteins, organism-specific biosystem; Late Phase of HIV Life Cycle, organism-specific biosystem; Nuclear import of Rev protein, organism-specific biosystem
• Function: DNA binding; Ran guanyl-nucleotide exchange factor activity; chromatin binding; guanyl-nucleotide exchange factor activity; histone binding; nucleosomal DNA binding; protein binding