Recombinant Human RASSF5 293 Cell Lysate

• Cat.No.: RASSF5-2495HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RASSF5 Ras association (RalGDS/AF-6) domain family member 5 [ Homo sapiens ]
• Official Symbol: RASSF5
• Synonyms: RASSF5; Ras association (RalGDS/AF-6) domain family member 5; ras association domain-containing protein 5; Maxp1; NORE1; RAPL; new ras effector 1; Rap1-binding protein; novel Ras effector 1; tumor suppressor RASSF3; Ras effector-like protein; Ras association (RalGDS/AF-6) domain family 5; regulator for cell adhesion and polarization enriched in lymphoid tissue; NORE1A; NORE1B; RASSF3; MGC10823; MGC17344
• Gene ID: 83593
• mRNA Refseq: NM_182663
• Protein Refseq: NP_872604
• MIM: 607020
• UniProt ID: Q8WWW0
• Chromosome Location: 1q31
• Pathway: Leukocyte transendothelial migration, organism-specific biosystem; Leukocyte transendothelial migration, conserved biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem; Pathways in cancer, organism-specific biosystem; TCR signaling in naive CD4+ T cells, organism-specific biosystem; TCR signaling in naive CD8+ T cells, organism-specific biosystem
• Function: metal ion binding; protein binding