Recombinant Human RAET1L protein, hFc-tagged

• Cat.No.: RAET1L-1388H
• Product Overview: Recombinant Human RAET1L protein(Q5VY80)(Arg26-Gly218), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: C-hFc
• Protein Length: Arg26-Gly218
• Tag: C-hFc
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human NKG2D, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 48.61 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.