Recombinant Human RAD1 Homolog (S. pombe), His-tagged

• Cat.No.: RAD1-2756H
• Product Overview: Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C.
• Purity: > 90% by SDS – PAGE.
• Sequences: MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES.
• Molecular Weight: 33.9 kDa (302aa)confirmed by MALDI-TOF.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RAD1 RAD1 homolog (S. pombe) [ Homo sapiens ]
• Synonyms:
  RAD1; RAD1 homolog (S. pombe); DNA repair exonuclease REC1; DNA repair exonuclease rad1 homolog; OTTHUMP00000115992; cell cycle checkpoint protein Hrad1; cell cycle checkpoint protein RAD1; checkpoint control protein HRAD1; exonuclease homolog RAD1; rad1-like DNA damage checkpoint protein; HRAD1; REC1; REC1; HRAD1;lEC 3.1.11.2
• Gene ID: 5810
• mRNA Refseq: NM_002853
• Protein Refseq: NP_002844
• MIM: 603153
• UniProt ID: O60671
• Chromosome Location: 5p13.2
• Pathway: Cell Cycle Checkpoints
• Function: 3"-5" exonuclease activity; damaged DNA binding; exodeoxyribonuclease III activity; hydrolase activity; protein binding