Recombinant Human PYGL

• Cat.No.: PYGL-31270TH
• Product Overview: Recombinant full length Human PYGL with N terminal proprietary tag. Predicted MW 119.24 kDa.

Specification

• Species: Human
• Source: Wheat Germ
• Tag: Non
• ProteinLength: 847 amino acids
• Description: This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Molecular Weight: 119.240kDa inclusive of tags
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.3% Glutathione, 0.79% Tris HCl
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTL VKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCP KRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDI EELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGI RYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLP VHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVN TMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGST RGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKL PWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRH LEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRI NMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKF QNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLT KLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINP SSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFV PRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSK LKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNM KFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVA ALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFK DIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNT MVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNE SNKVNGN
• Sequence Similarities: Belongs to the glycogen phosphorylase family.

Gene Information

• Gene Name: PYGL phosphorylase, glycogen, liver [ Homo sapiens ]
• Official Symbol: PYGL
• Synonyms: PYGL; phosphorylase, glycogen, liver; phosphorylase, glycogen; liver; glycogen phosphorylase, liver form; glycogen storage disease type VI; Hers disease
• Gene ID: 5836
• mRNA Refseq: NM_001163940
• Protein Refseq: NP_001157412
• MIM: 613741
• Uniprot ID: P06737
• Chromosome Location: 14q11.2-q24.3
• Pathway: Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem
• Function: AMP binding; ATP binding; bile acid binding; drug binding; glucose binding