Recombinant Human PYCARD Protein, Myc/DDK-tagged

• Cat.No.: PYCARD-01H
• Product Overview: Recombinant protein of human PYD and CARD domain containing (PYCARD), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 21.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: PYCARD PYD and CARD domain containing [ Homo sapiens (human) ]
• Official Symbol: PYCARD
• Synonyms: PYCARD; PYD and CARD domain containing; ASC; TMS; TMS1; CARD5; TMS-1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain-containing protein 5; target of methylation-induced silencing 1
• Gene ID: 29108
• mRNA Refseq: NM_013258
• Protein Refseq: NP_037390
• MIM: 606838
• UniProt ID: Q9ULZ3