Recombinant Human PYCARD Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: PYCARD-080H
• Product Overview: PYCARD MS Standard C13 and N15-labeled recombinant protein (NP_660184) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 15 kDa
• AA Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PYCARD PYD and CARD domain containing [ Homo sapiens (human) ]
• Official Symbol: PYCARD
• Synonyms: PYCARD; PYD and CARD domain containing; ASC; TMS; TMS1; CARD5; TMS-1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain-containing protein 5; target of methylation-induced silencing 1
• Gene ID: 29108
• MIM: 606838
• UniProt ID: Q9ULZ3