Recombinant Human PXN Protein, His-tagged

• Cat.No.: PXN-01H
• Product Overview: Recombinant Human PXN Protein (Asp2-Cys557) with a N-terminal 6x histidine tag was expressed in E. coli.

Specification

• Description: This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder
• Molecular Mass: 63 kDa
• Purity: > 95%
• Applications: ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array
• Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in 40 mM Tris-HCl (pH 8.0), 50 mM NaCl.
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
• Shipping: Ice Packs
• Protein length: 2-557 a.a.

Gene Information

• Gene Name: PXN paxillin [ Homo sapiens (human) ]
• Official Symbol: PXN
• Synonyms: PXN; paxillin; paxillin; testicular tissue protein Li 134
• Gene ID: 5829
• mRNA Refseq: NM_025157
• Protein Refseq: NP_079433
• MIM: 602505
• UniProt ID: P49023