Active Recombinant Human PVRL3 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: PVRL3-2187H
• Product Overview: Biotinylated Human Nectin-3, Fc Tag is expressed from HEK293 cells. It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 58-400 a.a.
• Predicted N Terminal: Leu 56 & Gly 58
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human TIGIT, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human Nectin-3, Fc Tag with a linear range of 0.102-4 ug/mL.
• Molecular Mass: The protein has a calculated MW of 66.1 kDa. The protein migrates as 90-95 kDa and 100-115 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL3 poliovirus receptor-related 3 [ Homo sapiens ]
• Official Symbol: PVRL3
• Synonyms: PVRL3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; CD113; CDw113; DKFZP566B0846; nectin 3; PPR3; PVRR3; PRR3; CDW113; NECTIN-3; FLJ90624; DKFZp566B0846
• Gene ID: 25945
• mRNA Refseq: NM_001243286
• Protein Refseq: NP_001230215
• MIM: 607147
• UniProt ID: Q9NQS3