Active Recombinant Human PVRL3 protein, Fc-tagged

• Cat.No.: PVRL3-0544H
• Product Overview: Recombinant Human PVRL3 protein(Gly58-Asp400), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 58-400 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Nectin-1, His Tag at 2 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag with a linear range of 5-78 ng/mL. Immobilized Human TIGIT, His Tag at 1 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag with a linear range of 0.156-2.5 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 64.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly58-Asp400
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL3 poliovirus receptor-related 3 [ Homo sapiens ]
• Official Symbol: PVRL3
• Synonyms: PVRL3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; CD113; CDw113; DKFZP566B0846; nectin 3; PPR3; PVRR3; PRR3; CDW113; NECTIN-3; FLJ90624; DKFZp566B0846
• Gene ID: 25945
• mRNA Refseq: NM_001243286
• Protein Refseq: NP_001230215
• MIM: 607147
• UniProt ID: Q9NQS3