Recombinant Human PTPRK protein, His & T7-tagged

• Cat.No.: PTPRK-1432H
• Product Overview: Recombinant Human PTPRK aa. (Thr1185~Ser1440 (Accession # Q15262)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Thr1185~Ser1440
• Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP was shown to mediate homophilic intercellular interaction, possibly through the interaction with beta- and gamma-catenin at adherens junctions. Expression of this gene was found to be stimulated by TGF-beta 1, which may be important for the inhibition of keratinocyte proliferation.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.3kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PTPRK protein tyrosine phosphatase, receptor type K [ Homo sapiens (human) ]
• Official Symbol: PTPRK
• Synonyms: R-PTP-kappa; dJ480J14.2.1 (protein tyrosine phosphatase, receptor type, K (R-PTP-KAPPA, protein tyrosine phosphatase kappa ,; protein tyrosine phosphatase kappa; protein-tyrosine phosphatase kappa; protein-tyrosine phosphatase, receptor type, kappa; receptor-type tyrosine-protein phosphatase kappa
• Gene ID: 5796
• mRNA Refseq: NM_001135648.2
• Protein Refseq: NP_001129120.1
• UniProt ID: Q15262