Recombinant Human PTPN1 293 Cell Lysate

• Cat.No.: PTPN1-2686HCL

Specification

• Description: Antigen standard for protein tyrosine phosphatase, non-receptor type 1 (PTPN1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: PTPN1 protein tyrosine phosphatase, non-receptor type 1 [ Homo sapiens ]
• Official Symbol: PTPN1
• Synonyms: PTPN1; protein tyrosine phosphatase, non-receptor type 1; PTP1B; tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
• Gene ID: 5770
• mRNA Refseq: NM_002827
• Protein Refseq: NP_002818
• MIM: 176885
• UniProt ID: P18031
• Chromosome Location: 20q13.1-q13.2
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; EGF receptor (ErbB1) signaling pathway, organism-specific biosystem; Growth hormone receptor signaling, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: ephrin receptor binding; hydrolase activity; insulin receptor binding; protein binding; protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; zinc ion binding