Recombinant Human PTK2B Protein Tyrosine Kinase 2 Beta, His-tagged

Cat. No. :	PTK2B-1904H
Description :	PTK2B is a cytoplasmic tyrosine kinaseinvolved in calcium-induced regulation of ion channels and activation of themap kinase signaling pathway. No special measures were taken to activate thiskinase.
Source :	Sf9 insect cells
Species :	Human
Form :	Liquid in 25 mMTris-HCl, pH 8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
Molecular Weight :	120.0 kDa
Purity :	> 90% asdetermined by SDS-PAGE analysis
Biological Activity :	20 U/mg. One unit isdefined as the amount of enzyme that will phosphorylate 1 nmol of tyrosinesubstrate per minute at pH 7.4 and at 30°C. Assay buffer: 50 mM HEPES, pH 7.4+ 3 mM MgCl2 + 3 mM MnCl2 + 1 mM DTT + 3 mMNa-orthovanadate + 0.5 mM ATP + 30 ug/ml Poly (Glu:Tyr) 4:1 substrate + 4 ug/mlrecombinant PTK2B.
Storage :	Stablefor 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol :	PTK2B
Full Length :	Full L.

Gene Name	PTK2B PTK2B protein tyrosinekinase 2 beta [ Homo sapiens ]
Synonyms	PTK2B; PTK2B protein tyrosinekinase 2 beta; PTK2B protein tyrosine kinase 2 beta; CAK beta;calcium-dependent tyrosine kinase; cell adhesion kinase beta; focal adhesionkinase 2; proline-rich tyrosine kinase 2; protein kinase B; protein tyrosinekinase 2 beta; related adhesion focal tyrosine kinase; EC 2.7.10.2
Gene ID	2185
mRNA Refseq	NM_004103
Protein Refseq	NP_004094
MIM	601212
UniProt ID	Q14289
Chromosome Location	8p21.1
Pathway	Calcium signaling pathway;Chemokine signaling pathway; GnRH signaling pathway; Leukocytetransendothelial migration; Natural killer cell mediated cytotoxicity
Function	3-phosphoinositide-dependentprotein kinase binding; ATP binding; non-membrane spanning protein tyrosinekinase activity; nucleotide binding; protein complex binding; signaltransducer activity; transferase activity