Recombinant Mouse Ptk2b Protein, Myc/DDK-tagged

• Cat.No.: Ptk2b-5227M
• Product Overview: Purified recombinant protein of mouse full-length PTK2 protein tyrosine kinase 2 beta (Ptk2b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 115.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ptk2b PTK2 protein tyrosine kinase 2 beta [ Mus musculus (house mouse) ]
• Official Symbol: Ptk2b
• Synonyms: PTK2B; PTK2 protein tyrosine kinase 2 beta; protein-tyrosine kinase 2-beta; FADK 2; CAK-beta; focal adhesion kinase 2; cell adhesion kinase beta; cellular adhesion kinase beta; proline-rich tyrosine kinase 2; protein tyrosine kinase 2 beta; calcium-dependent tyrosine kinase; related adhesion focal tyrosine kinase; calcium-regulated non-receptor proline-rich tyrosine kinase; CAKB; FAK2; PYK2; CADTK; FADK2; Raftk; CAKbeta; E430023O05Rik
• Gene ID: 19229
• mRNA Refseq: NM_001162366
• Protein Refseq: NP_001155838
• UniProt ID: Q9QVP9