Recombinant Human PSME3 protein, His-tagged

• Cat.No.: PSME3-359H
• Product Overview: Recombinant Human PSME3 (Met1-Tyr254) protein was fused to His-tag at N-terminus and expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: Met1-Tyr254
• Description: Proteasome activator complex subunit 3 (PSME3),a member of the PA28 family, is also known as 11S regulator complex subunit gamma (REG-gamma), activator of multicatalytic protease subunit 3 and proteasome activator 28 subunit gamma (PA28gamma). PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSME3 can also facilitate the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Furthermore,PSME3 may also be involved in cell cycle regulation.
• Predicted N Terminal: Met1
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 30.3 kDa. The protein migrates as 28-33 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PSME3
• Official Symbol: PSME3
• Synonyms: PSME3; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); proteasome activator complex subunit 3; Ki; PA28 gamma; PA28G; REG GAMMA; PA28gamma; Ki antigen; Ki nuclear autoantigen; proteasome activator 28-gamma; 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; proteasome activator 28 subunit gamma; activator of multicatalytic protease subunit 3; REG-GAMMA; PA28-gamma
• Gene ID: 10197
• mRNA Refseq: NM_005789
• Protein Refseq: NP_005780
• MIM: 605129
• UniProt ID: P61289