Recombinant Human PSMD4, GST-tagged

• Cat.No.: PSMD4-152H
• Product Overview: Recombinant human PSMD4 (amino acid residues 1-377), with N-terminal GST, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-377 a.a.
• Description: S5a (Rpn10) is a major ubiquitin binding protein that binds preferentially to polyubiquitin chains. It is found as a subunit of the 26S proteasome, but unlike other proteasome subunits, S5a also exists predominantly as a free protein in the cytosol. S5a contains two stretches of approximately 15 amino acids called the ubiquitin interacting motifs (UIMs), which are responsible for its high affinity for ubiquitin chains (Kim and Goldberg, 2012).
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Molecular Mass: ~67.6kDa
• Purity: >98% by InstantBlue™ SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 [ Homo sapiens ]
• Official Symbol: PSMD4
• Synonyms: PSMD4; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; 26S proteasome non-ATPase regulatory subunit 4; AF; AF 1; Rpn10; S5A; angiocidin; RPN10 homolog; antisecretory factor 1; S5a/antisecretory factor protein; multiubiquitin chain-binding protein; 26S proteasome regulatory subunit S5A; ASF; AF-1; MCB1; pUB-R5
• Gene ID: 5710
• mRNA Refseq: NM_002810
• Protein Refseq: NP_002801
• MIM: 601648
• UniProt ID: P55036
• Chromosome Location: 1q21.2
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem
• Function: protein binding